Here, we highlight three important process considerations across the RNA-LNP manufacturing workflow which includes limit size behavior, in-line dilution and downstream tangential flow filtration (TFF).
By performing a small-scale study with multiple microcarrier types and medias we identify the best microcarrier and media for the expansion of porcine SK-RST cells via microcarrier-based serial passage in stirred vessels.
In this application note we characterize MRC-5 cell growth on multiple microcarriers in stirred vessels and present data demonstrating feasibility of serial passage and scale-up into bioreactor expansion of MRC-5 cells.
We demonstrate how microcarriers provide an ideal substrate for expansion of MDCK cells in closed stirred-vessel systems and lay the groundwork for subsequent developmental studies in larger bioreactor formats.
We explore a unique single-use technology platform fitting all mixing applications from buffer and media preparations, downstream processes to final formulation in 50 L, 100 L, 200 L, 400 L, 650 L and 1,000 L volumes.