Refocusing R&D strategies towards diagnostics and considering industry partnerships should be key considerations when developing enterprise risk plans during a crisis like COVID-19. 

qPCR remains the gold standard for validation of microarray and next generation sequencing data and the method of choice for both clinical and basic research labs for a wide range of applications. However, there remains general concern about the production of data that truly reflects the tested experimental conditions. We have developed a comprehensive guide to performing the ultimate qPCR experiment. The following is a snapshot of the critical steps needed to achieve excellent results.

RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics, as well as food safety. There are multiple benefits of this technology. In this application note, we demonstrate a sensitive codetection of viral RNA and DNA targets in a multiplex setting using a novel one-step multiplex RT-qPCR supermix.

Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability. Learn more about the CFX Real-Time PCR Detection Systems which uses a simple protocol to measure protein thermal stability using SYPRO Orange Fluorescent Dye.

As pharmaceutical industry faces pressure to bring new drugs to market more quickly and at minimal cost, pharmaceutical and biotechnology companies are increasingly outsourcing various parts of the drug development and manufacturing process. This white paper highlights six key points that pharmaceutical and biotechnology companies should keep in mind when looking to select the ideal partner to synthesize an API for them—and ensure their drug development process stays on the path to success.

Reference gene selection and validation are very important for gene expression studies that involve different samples or experimental conditions and therefore are absolutely necessary before any further exploration. Read how PrimePCR Reference Gene Selection Panels provide an easy-to-use system with which to select appropriate reference genes.

Stain-free technology, a technology that existed in principle since early 2000 but was only commercialized in 2010 has changed the protein separation and analysis landscape in the past few years. This article focuses on how protein visualization is accomplished using stain-free technology, its advantages and concerns as well as how stain-free technology can change the way we carry out protein separation and analysis.

Process characterization and validation can be challenging, but using a risk-based approach to generate an appropriate control strategy will help assure your product meets these requirements, enabling timely approval and launch to market.

When working with limited patient samples, the ability to test multiple parameters simultaneously is highly valuable. This application note reviews how to successfully monitor in-depth changes to the T cell phenotype, exhaustion, and cellular subset identity markers using a 16-parameter panel developed for use with a 5-laser ZE5 Cell Analyzer. Using an in vitro model, they examined T cells before and after chronic stimulation, and in combination with a PD-1 receptor inhibitor, nivolumab.

CRISPR's speed, low cost, and ability to be used in many model systems redefine what is possible in drug discovery and development. However, current workflows  for generating knockout cell lines using CRISPR-Cas9 can be complex and inefficient. In this study, we demonstrate a novel workflow that includes Droplet Digital™ PCR, high resolution melt analysis, western blotting, and real-time PCR to generate and validate a knockout haploid cell line in less than five weeks.