When working with limited patient samples, the ability to test multiple parameters simultaneously is highly valuable. This application note reviews how to successfully monitor in-depth changes to the T cell phenotype, exhaustion, and cellular subset identity markers using a 16-parameter panel developed for use with a 5-laser ZE5 Cell Analyzer. Using an in vitro model, they examined T cells before and after chronic stimulation, and in combination with a PD-1 receptor inhibitor, nivolumab.

CRISPR's speed, low cost, and ability to be used in many model systems redefine what is possible in drug discovery and development. However, current workflows  for generating knockout cell lines using CRISPR-Cas9 can be complex and inefficient. In this study, we demonstrate a novel workflow that includes Droplet Digital™ PCR, high resolution melt analysis, western blotting, and real-time PCR to generate and validate a knockout haploid cell line in less than five weeks.

Genome editing has become a widely used tool and allows specific modification of almost any cell’s genome. However, the often low (<5%) frequency of gene edits, in particular for some primary or induced pluripotent stem cell types, hinders facile and reliable detection by gel- or sequencing-based methods, or requires laborious clonal isolation of edited cells. Here we introduce an ultra-sensitive, cost-efficient Droplet Digital PCR–based method for the detection of HDR and NHEJ alleles generated using the CRISPR/Cas9 and TALEN genome editing systems.

Realizing the benefits of process characterization requires proper planning and application of a comprehensive process characterization strategy.

Accelerating the production of your viral-vector based therapy requires you to understand the strategies available to address the viral vector manufacturing shortage as well as selecting the most cost-effective solution for advancing your therapeutic.

Catch up on the current opportunities and challenges for using mechanistic modeling to simulate and predict chromatographic behavior and experiments in silico. 

Efficient monitoring of T cell surface markers, cytokines, and expression patterns is critical to the study of immunotherapies. Learn how the Ze5 Cell Analyzer enables you to test these multiple parameters simultaneously.

Downstream processes for biomolecule purification are becoming more complex. Challenges include increasing titers from upstream cell culture, greater diversity of target molecules, and a need for better productivity. HIC has characteristics designed to solve these challenges. In this study, the hydrophobicities of newer generation Capto HIC resins were compared to Sepharose High Performance and Sepharose Fast Flow HIC resins.

The supply chain and logistics for cell and gene therapies are especially critical to ensure the right patient gets the right treatment at the right time. Collaboration is the key to solving challenges such as data management and process variability, as stakeholders work towards a common goal—getting these life-saving treatments to the people who need them.

The cell and gene therapy industry can move faster by shoring up its weakest links. Q&A with GE Healthcare’s Phil Vanek about how to do it.