The Importance Of Finding The Right Media For Your CHO Subtype

There are now various cell lines derived from the original CHO cells, including CHO-K1, CHO-DXB11, CHO-DG44, CHO-S, and CHO-GS. Each has slightly different attributes, growth requirements, and responsiveness to environmental conditions. Therefore, the choice of media can strongly influence their performance. Their history is lengthy and often confusing.

There is substantial genetic heterogeneity among the different CHO cell lineages, creating cell line-specific differences in cell behavior, nutrient requirements, favorable culture conditions, protein production capabilities, and product quality. To further complicate things, the cell culture medium also significantly impacts cell performance and product quality. But this also provides an opportunity to optimize the performance of your specific CHO cell line.

A media benchmarking is necessary to find a truly compatible medium for your unique cell line. After finding a compatible medium, testing different feeding strategies can further enhance cell growth and productivity. A one-size-fits-all approach might yield satisfactory results, but you might achieve significant improvements in cell performance by employing a more tailored strategy. Learn more about the origin of CHO cell lines and how testing different media can help optimize your upstream production process.