What is tagged protein purification?
Tagged protein purification uses affinity chromatography (AC) to purify recombinant proteins that have been engineered to include a specific peptide or protein sequence (tag).
The use of tags significantly simplifies purification and enables use of standard protocols.
How does it work?
The target protein, with an affinity tag attached, is specifically and reversibly bound to a chromatographic resin containing a binding substance (ligand) with affinity to the tag.
This paper details the ÄKTA go. ÄKTA go is a small and compact liquid chromatography system that allows researchers to perform routine protein purification with ease while allowing for efficient use of bench and cold cabinet space.