ActiPro medium and supplements were developed to support high productivity of CHO cells in biomanufacturing of recombinant proteins, and the products have delivered excellent results with various CHO cell lines (1). ActiPro production medium and Cell Boost feed supplements are intended to be used together, following an easy-to-use starting protocol. The feed supplements are designed to complement each other and the recommended ratio of Cell Boost 7a to 7b is 10/1 (v/v). As a start, recommended feed volumes of Cell Boost 7a and 7b are 3% and 0.3%, respectively, of the total culture volume. In previous experiments, this feeding strategy was also shown to be optimal for a high-titer mAb-producing CHO DG44 cell line (internal data). However, the feed volumes should be adjusted with respect to the nutritional requirements of the specific cell clone used. In this work, a feeding strategy was developed in shake flasks for a CHO-S cell line producing a monoclonal antibody in lower titers. The productivity was further optimized in bioreactor cultures using ReadyToProcess WAVE™ 25 bioreactor system.