Cell Line And Upstream Process Development Services

Cellca CHO Expression Platform

Sartorius is a leading provider of Cell Line Development Services allowing customers easy open access to a cost effective reliable technology platform. The Cellca CHO Expression Platform consistently delivers well characterized stable research clones from DNA to Research Cell Bank (RCB) in just 14 weeks with average titers of 4 g/L in an easily scalable fed batch process.

DHFR selection system w|o MTX amplification

Host Cell Line

• CHO DG44 growth in suspension
• 18 h doubling time
• Long term stability
• Host cell line fully documented & tested according to ICH & WHO

Expression Vector

• Metabolic Selection
• Low DHFR expression
• S/MAR elements
• Optimal signal peptide & promoter
• Freedom-to-operate

Media System

• Optimized for Cellca cell lines
• Chemically defined, free of animal components and proteins
• High cell densities, viabilities & productivity

Upstream Process Design

• Robust, east to transfer to client/CMO
• Easy to scale, proven performance in various bioreactor systems
• Ready-to-use protocol