Benchmarking Filtration Performance Of Minimate™ Tangential Flow Filtration Cassettes

Tangential flow filtration (TFF) is a widely used and cost-effective technique for concentrating and purifying proteins through processes such as diafiltration and ultrafiltration. Unlike traditional filtration methods, TFF operates as a continuous process, utilizing a semi-permeable membrane to separate molecules based on their size. This method is highly versatile, capable of handling sample volumes ranging from as little as 10 milliliters to several thousand liters, which makes it suitable for both laboratory-scale and industrial applications. In this application note, our investigation focused on comparing the filtration flux, membrane permeability, and protein retention characteristics of the Minimate cassettes with those of Sartorius Vivaflow 50 laboratory cross-flow cassettes that use regenerated cellulose membranes. Furthermore, we explore how increasing protein concentration affects filtration performance, particularly in relation to flux rates and the selection of an appropriate molecular weight cut-off (MWCO).

Learn more about this analysis and gain insight into how to optimize your TFF systems for efficient protein processing at a laboratory scale.