Application Note

Tuning Intermediate Volumes On Sepax™ C-Pro Cell Processing System

Source: Cytiva
GettyImages-924655686-lab-cell

Optimizing cell harvesting in a closed and automated manner is important when it comes to reducing processing time for expanded cell therapy products, and can help quickly deliver treatments to patients. We offer the CultureWash C-Pro application software for the SepaxTMC-Pro System to perform automated processing, maximize cell recovery, and minimize operator intervention while avoiding open, manual techniques. Using a model human B-lymphoblastoid cell line, we tested a predictive mathematical formula for calculating minimum intermediate volume that is applicable up to 4 billion total cells for a final product dose of 20 mL, and suggest an optimal parameter set for the CultureWash application when above this cell number. Discover how we automate the process and further minimize the potential for cell loss during wash steps.

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