The MIRO CANVAS NGS Prep System Enables Fully Automated Library Prep For Nanopore Human Whole Genome Sequencing (WGS)

Advances in long‑read sequencing are expanding what’s possible in whole‑genome analysis, and fully automated library preparation is becoming essential for labs seeking consistency and scale. This application note explores an automated high‑input workflow for preparing human whole‑genome libraries using HMW DNA and the latest ligation sequencing chemistry. The workflow supports inputs up to 5 μg and accommodates fragment sizes above 30 kb while significantly reducing reagent use through miniaturized reaction volumes. Sequencing data from 10 kb and 30 kb samples demonstrates strong alignment between automated and manual preparation, including comparable read lengths, >30× genome coverage, >Q20 accuracy, and high pore occupancy. Variant‑calling metrics—spanning SNVs, INDELs, structural variants, and clinically relevant SV‑CMRGs—underscore the workflow’s reliability for downstream analysis.

Dig into the full application note to examine performance data, workflow details, and comparative sequencing results.