Sartoclear® Depth Filters For The Clarification Of AAV

By Marcel Fueger, Yeonhwa Jin, Axel Thiefes, and Michal Szelwicki

Depth filtration plays an essential role in producing cell and gene therapies, particularly in clarifying adeno-associated virus (AAV) vectors. This study assessed the effectiveness of Sartoclear® depth filters in processing AAV8 vectors derived from HEK293 cell cultures.

Key Findings:

• Exceptional Performance: Small-scale experiments demonstrated high filtration capacity, excellent protection for downstream sterilizing filters, and strong product recovery rates.
• Scalability: Process-scale evaluations highlighted the robust and scalable performance of Sartoclear® DL75 filters, ensuring high recovery even with challenging feed materials.

Implications:

The use of Sartoclear® filters in suspension-based AAV processes enhances purification efficiency and product yield, representing a significant advancement in supporting the broader accessibility of cell and gene therapies for patients.