Article | October 13, 2022

Optimizing Capture And Polishing Steps In An rAAV Purification Process

Source: Cytiva

Scalable, cost-efficient, and robust filtration and chromatography-based processes are required for the purification of adeno-associated virus (AAV) in bioprocessing.

The key factors for an effective AAV purification process are high overall yields and maximized empty capsid product impurity reduction. After harvest by detergent cell lysis and DNA degradation by nuclease treatment, the feed is clarified followed by a concentration and buffer exchange step that reduces the volume to prepare for the capture step. Affinity capture is an efficient way to specifically bind and concentrate the target and at the same time achieve a high impurity removal.

We adapted an affinity capture step for rAAV2 and rAAV5, followed by a polishing step with rAAV5 to improve the yield of rAAV full capsids.

In this article, we show how we developed an efficient purification process for recombinant AAV (rAAV). The chromatography purification described includes affinity capture to maximize recovery with efficient impurity removal.


Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Drug Discovery Online? Subscribe today.

Subscribe to Drug Discovery Online X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.


Subscribe to Drug Discovery Online