Application Note

Lysate Clearance To Remove Cellular Debris

Molecular structure iStock-1263966452

Molecular biology methods have led to the successful production of a variety of synthesized biomolecules (DNA, RNA, proteins) from prokaryotic and eukaryotic cell lines. The most problematic step in the purification of these biomolecules is the clarification of the sample once the cells are lysed. The lysate often contains biomolecule concentrations millions of times higher than the molecule of interest.

In the past, centrifugation was the primary method used to sediment cellular debris. Sedimentation has several limitations for many applications where small-scale, high-throughput processing is required.

Filtration, which is easily automated, is relatively quick and allows the use of additional wash steps to maximize sample recovery. Filtration can be done effectively in either vacuum or centrifugal mode, ultimately maximizing the choice in protocols available to the researcher.

We present a lysate sample preparation procedure using the AcroPrep™ Advance filter plate for lysate clearance that effectively removes unwanted cellular debris from samples.


Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Drug Discovery Online? Subscribe today.

Subscribe to Drug Discovery Online X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.


Subscribe to Drug Discovery Online