By Gerco van Eikenhorst, Bella Monica, Roni Hazan Brill, Emilie Rodrigues, and Yvonne Thomassen
Cell culture media, including currently available animal component free media, contain undefined polypeptides. To optimize the cell and virus culture it is desirable to know which nutrients are depleted at the different cultivation phases. However, media containing undefined ingredients make this more challenging. Therefore, a chemically defined medium is needed. In collaboration with Biological Industries the development of a chemically defined (BiCD) medium for the growth of Vero cells, and subsequent virus production, was perused. The developed medium is chemically defined and animal component free, it supports Vero cell growth in static and bioreactor cultures and is able to support propagation of different type of viruses.