CHO Media Screening Success Using Benchmarking Studies

By Dennis Karthaus, Catherine Krikelis, Swapnil Chaudhari, and Dirk Mueller

Recombinant proteins account for more than half of the revenue in the biopharmaceutical industry. This competitive landscape, coupled with pressures from government agencies to improve access to effective and affordable medicine, requires recombinant protein manufacturers to enhance production efficiency while reducing costs.

A key opportunity to maximize productivity lies in optimizing the upstream process, which supplies the material for the rest of production. The journey toward reliable and productive processes begins with selecting the right cell culture conditions and matrix. However, determining the optimal media and feed combination, as well as the appropriate feeding regime can be complex and time-consuming.

This white paper outlines the challenges of media screening and proposes a straightforward approach for saving time, reducing risks, and improving outcomes. Additionally, a case study demonstrating a method for performing reliable media screening is presented.