Characterize Your PROTAC Hook Effect With Flexible And In-Solution Affinity Measurements For Results You Can Trust

After assembling promising PROTAC candidates, the next critical step is characterizing the ternary complex hook effect. To select candidates that will succeed in vivo, it’s important to favor those that enhance cooperativity—a proven approach for mitigating the hook effect by improving ternary complex stability.

The Dianthus platform, using Spectral Shift technology, provides a robust method for these characterizations. It performs these crucial affinity measurements in-solution, removing the need to immobilize any of the ternary complex components.

This application note details the characterization of a highly cooperative complex, determining binary affinity, the PROTAC concentration for maximum complex formation, and a positive cooperativity value (α) known to correlate with efficient target degradation. Learn how this flexible assay setup can give you confidence in your lead candidates.