Automated Library Preparation For A Nanopore DNA Barcoding Protocol On The MIRO CANVAS

Automating long‑read sequencing workflows can dramatically reduce hands‑on time while preserving data quality, and this protocol demonstrates how digital microfluidics makes that possible. By integrating key steps of the native DNA barcoding workflow—bead clean‑ups, adapter ligation, and final library preparation—into a compact, portable system, researchers can prepare multiplexed samples with 50% less reagent volume and consistent sequencing performance. The method supports high‑molecular‑weight DNA, enabling reliable detection of multiple species in mixed communities and maintaining read length and quality comparable to manual preparation. With balanced barcode representation, robust read‑length distributions, and strong performance across mock microbial standards, this approach offers a streamlined option for labs aiming to scale long‑read sequencing or take sequencing workflows into the field.

Explore the full application note to see the workflow, performance metrics, and species‑level results in detail.