Agilent Combines HPLC, Microplates for High-Throughput Analysis

Agilent Technologies Inc. (Palo Alto, CA) has introduced a fully automated, integrated hardware and software system that speeds the collection, analysis, confirmation, and purification of chemical samples. With the capacity to handle and store over 4,600 samples unattended, it offers increased throughput for drug discovery, development, and new drug testing. The system should be available worldwide this month.

Agilent has combined its 1100 Series high-performance liquid chromatography models with the Agilent 220 micro plate sampler and software to create an integrated high-throughput sample processor. Adding an LC/MSD mass spectrometer provides molecular weight confirmation and makes possible mass-based fraction collection, sample purification, and purity verification.

Using the same microplate sampler for 96- and 384-well plates, for both sampling and fraction collection, allows users to analyze samples; purify a single component based on its molecular weight, and then re-analyze it to verify purity. The system can perform mass-based fraction collection, as well as peak-based or time-base collection. Timing can be determined using the delay sensor and chemicals included with the system, enabling faster method development.

A new flow injection analysis mode improves throughput for samples that do not require chromatographic separation. The contents of a 96-well plate can be processed in less than one hour. And for convenience, the results can be stored in a single data file.

For more information: Lois Replenski, Agilent Technologies Ltd., 2850 Centerville Rd., Wilmington, DE 19808. Tel: 800-227-9770 or 302-633-8247.

Edited by Angelo DePalma