Accelerating Cell Therapy Discovery With High-Throughput Electroporation

When engineering CRISPR-mediated CAR-T cells, the sheer number of variables to optimize, including guide RNA sequences, nuclease selection, and HDR template concentration, can slow your path from discovery to the clinic.

This application note walks you through a high-throughput electroporation screening workflow that evaluates up to 96 conditions in parallel, achieving greater than 99% TRAC knockout and greater than 72% CD19 CAR knock-in while preserving cell viability. See how optimized conditions transfer directly to a larger-scale instrument without re-optimization, with consistent CAR expression and cell yields confirmed across three healthy donors.

Access the application note below to evaluate how this approach fits your development strategy.