A Transient Cell Line To Produce rAAV With Low-Level hcDNA Encapsidation

Recombinant adeno-associated virus (rAAV) manufacturing is undergoing a significant transformation, with stable producer cell lines emerging as the primary cell factories of the future. However, transitioning to producer cell lines is a nuanced process, necessitating the utilization of transient transfection cell lines that prioritize speed and flexibility. To meet these requirements, a novel human embryonic kidney (HEK) 293 suspension cell line has been developed. The line ELEVECTA™ transient cell line is designed to integrate seamlessly into diverse research workflows and large-scale GMP production. Here, we showcase its ability for efficient and scalable rAAV production across various serotypes with low encapsidated host cell DNA (hcDNA) levels.