Cytiva product datasheets, catalogs, app notes

  1. Seed Culture Expansion Process: Reduce Time And Maximize Utilization 12/15/2016

    This application note describes how perfusion cell culturing can be used to reduce processing time, simplify operations, and maximize equipment utilization in seed culture expansion processes. For creation of high-density cell banks, Chinese hamster ovary (CHO) cells were expanded using a bioreactor system operated in perfusion mode.

  2. Resolving Bottlenecks of Recombinant Antibody Fragments 6/6/2016

    Antibodies and their derivatives are increasingly important and essential tools for biological research. Techniques to screen and characterize large numbers of antibodies and provide confident selection of the best candidates for development are therefore essential. Details described in this method will provide rapid screening in combination with data-rich selection of fragments with high affinity and the desired kinetic behavior.

  3. Microbial Fermentation In Single-Use Xcellerex™ XDR-50 MO Fermentor System 10/24/2013

    This application note describes the performance of a single-use Xcellerex XDR-50 bioreactor system when used in cultivations of E. coli and of modified Pseudomonas fluorecsens (P. fluorescens) producing a monoclonal antibody (MAb). Both the achieved microbe densities and product yield were shown to be consistent with the performance of conventional stainless steel systems.

  4. Obtaining Good Yields With High Purity Of An N-terminal Histidine-Tagged Protein 10/16/2013

    ÄKTA start eliminates the hassles of manual protein purification handling, such as loading large sample volumes and continually monitoring UV readings, and can give you consistency of yield and purity. Madhu demonstrates how ÄKTA start aided him in purifying a recombinant N-terminal histidine-tagged protein.

  5. Automating Protein Purification Processes Achieves Reliable Results And Reproducibility 10/16/2013

    ÄKTA start is an easy-to-use, reliable protein purification system. In this application note it is described how Erica, a post-doctoral researcher, used ÄKTA start to purify GST-tagged protein using a predefined method template, a GSTrap FF column, and a Frac30 fraction collector.