Cytiva Cell datasheets catalogs app notes

  1. Optimizing Recovery On The Sepax™ Cell Processing System 7/21/2022

    Using a model T-cell line, we demonstrate the impact of varying rinse volume, intermediate volume, and manual extraction parameters on overall cell recovery with a final product dose volume of 20 mL.

  2. Genome Editing of Human Primary T Cells With Lipid Nanoparticles 4/18/2022

    We show CRISPR/Cas9-mediated knockouts of the T cell receptor and explore multi-step LNP engineering to produce TCRɑβ KO CAR T cells, a promising approach towards allogeneic CAR T cell therapy.

  3. Low-Volume Production For Screening High-Value Nanoparticle Materials 4/18/2022

    A platform that harnesses microfluidic mixing has been demonstrated as a simple, robust, and scalable production method for LNPs encapsulating various types of nucleic acids.

  4. Liquid Nitrogen-Free Cryopreservation For Sustainability 4/5/2021

    Discover the environmental implications of using liquid nitrogen for cell freezing, along with carbon emissions during the manufacture, transportation, and operation of LN2-free controlled-rate freezers.

  5. Upstream Platform For Continuous Lentiviral Vector Production 12/1/2020

    Here, we demonstrate a high-yield LV production process, using animal-derived component-free media and reagents, where multiple or continuous vector harvests could be obtained from a single batch.

  6. CAR-T Cell Lentiviral Transduction By Spinoculation 5/21/2020

    There is a growing need to close and automate manufacturing of chimeric antigen receptor (CAR)-T cell therapies. Learn how to streamline this step while keeping flexibility during process development.

  7. Controlled Endpoint In Cryopreservation 2/24/2020

    Successful cryopreservation of cells requires a controlled cooling rate. Cooling too rapidly or too slowly could lead to a poorer outcome. This application note demonstrates how controlled-rate cooling is achieved using equipment such as VIA Freeze controlled-rate freezers.

  8. The Effect Of Rocking Rate And Angle On T Cell Cultures Grown In Expansion Systems 2/23/2020

    Ensuring optimal and maximal T cell production is critical for adoptive immunotherapy and its continued success. The Xuri Cell Expansion System is an important component of the clinical manufacturing process so we sought to investigate the effect of the rocking rate and angle on the expansion of T cells.

  9. Large Volume Cryopreservation: A Liquid Nitrogen Free Proof-Of-Concept 6/6/2019

    This study demonstrates a cell freezing protocol that uses the LN2-free VIA Freeze Quad controlled-rate freezer for the cryopreservation of up to eight cryobags and up to 560 mL total volume.

  10. Water-Free Vs Water Bath Cell Thawing 11/29/2018

    This benchmarking study compares the performance of the VIA Thaw CB1000 system and a water bath for thawing frozen cells.