Cytiva Cell datasheets catalogs app notes
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Genome Editing of Human Primary T Cells With Lipid Nanoparticles
4/18/2022
We show CRISPR/Cas9-mediated knockouts of the T cell receptor and explore multi-step LNP engineering to produce TCRÉ‘β KO CAR T cells, a promising approach towards allogeneic CAR T cell therapy.
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Low-Volume Production For Screening High-Value Nanoparticle Materials
4/18/2022
A platform that harnesses microfluidic mixing has been demonstrated as a simple, robust, and scalable production method for LNPs encapsulating various types of nucleic acids.
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Liquid Nitrogen-Free Cryopreservation For Sustainability
4/5/2021
Discover the environmental implications of using liquid nitrogen for cell freezing, along with carbon emissions during the manufacture, transportation, and operation of LN2-free controlled-rate freezers.
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Upstream Platform For Continuous Lentiviral Vector Production
12/1/2020
Here, we demonstrate a high-yield LV production process, using animal-derived component-free media and reagents, where multiple or continuous vector harvests could be obtained from a single batch.
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CAR-T Cell Lentiviral Transduction By Spinoculation
5/21/2020
There is a growing need to close and automate manufacturing of chimeric antigen receptor (CAR)-T cell therapies. Learn how to streamline this step while keeping flexibility during process development.
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Controlled Endpoint In Cryopreservation
2/24/2020
Successful cryopreservation of cells requires a controlled cooling rate. Cooling too rapidly or too slowly could lead to a poorer outcome. This application note demonstrates how controlled-rate cooling is achieved using equipment such as VIA Freeze controlled-rate freezers.
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The Effect Of Rocking Rate And Angle On T Cell Cultures Grown In Expansion Systems
2/23/2020
Ensuring optimal and maximal T cell production is critical for adoptive immunotherapy and its continued success. The Xuri Cell Expansion System is an important component of the clinical manufacturing process so we sought to investigate the effect of the rocking rate and angle on the expansion of T cells.
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Closed And Semi-Automated Processing Of CAR T Cells
7/5/2019
Learn about a robust CAR T-cell manufacturing workflow that can be adapted for cGMP compliance in the commercial production of CAR T-cells.
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Alternative To Cryobags For Large Volume Cryopreservation
6/6/2019
This study demonstrates an optimized cooling protocol for cryopreserving large-volume cryovials in a controlled-rate freezer, avoiding the need for liquid nitrogen.
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Large Volume Cryopreservation: A Liquid Nitrogen Free Proof-Of-Concept
6/6/2019
This study demonstrates a cell freezing protocol that uses the LN2-free VIA Freeze Quad controlled-rate freezer for the cryopreservation of up to eight cryobags and up to 560 mL total volume.