Application Note

CAR-T Cell Lentiviral Transduction By Spinoculation

Source: Cytiva
Jumpstart Cell & Gene Therapy Commercialization With SUT Learning

Study overview: Automated spinoculation for gene transfer by lentiviral transduction

There is a growing need to close and automate manufacturing of chimeric antigen receptor (CAR)-T cell therapies. CAR-T workflows depend on ex vivo gene transfer by lentiviral transduction for therapeutic efficacy. To streamline this step while keeping flexibility during process development, we introduced stand-alone protocol software that uses existing Sepax C-Pro technology.

SpinOculation C-Pro protocol software automates lentiviral vector (LVV) transduction and maintains a functionally-closed system within a single-use disposable kit; importantly, no transduction enhancers are required. We present different use cases highlighting the flexibility of the SpinOculation C-Pro protocol software parameters for process optimization of LVV transduction efficiencies in combination with application-specific upstream processes and viral sources.

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