Snuggle Wrap-Based Restraint Method For Continuous Infusion In Juvenile Nonhuman Primates

By Narine Lalayeva, Gretchen Aylor, Jennifer Reynolds, and Julie Forget

As the use of pediatric pharmaceuticals in preclinical studies increases, it becomes essential to adapt testing methods to accommodate younger, smaller test systems while minimizing stress on research animals. Traditionally, infusion restraint at our facility involved using a procedure cage, a method that proved to be highly stressful for animals under a year of age and unsuitable for smaller primates. To address this, we developed the “snuggle wrap,” a technique that allows young animals to remain immobile while providing comfort through a hugging-like sensation. Recognizing the importance of maintaining social bonds, the "snuggle board" was also created. This device securely holds four animals in close proximity, ensuring that social pairs remain in visual and auditory contact during dosing.

In two 13-week studies involving intravenous infusions, this snuggle method was successfully used with animals ranging from 6 to 9 months, 12 to 15 months, and 40 to 48 months of age. The data from both the snuggle method and the procedure-cage method were pooled for comparison, and no significant differences were observed between the two restraint techniques. Consequently, both methods can be effectively employed depending on the age and size of the animals, with the snuggle method proving most suitable for younger primates.