Intensification Of mAb Processes: Leveraging Sartobind® Rapid A And A Fully Connected Membrane-Based DSP

By Fabien Rousset

Monoclonal antibody (mAb) purification requires high-performance, ready-to-use chromatography membranes compatible with a "one-batch-one-device" approach. Sartobind® Rapid A, a Protein A capture technology, achieves 10 times higher productivity than traditional resins (203 g/L vs. 14 g/L) while maintaining equivalent dynamic binding capacity (DBC), yield, and HCP/hcDNA removal efficiency.

This technology enables next-generation membrane-based purification platforms, including a competitive double-flowthrough polishing process with Sartobind® Q and Sartobind® S membranes. This method achieves over 98% purity and yield per step while reducing the overall process footprint.

To further advance fully membrane-based workflows, combining Resolute® MCC multi-column technology with Protein A, AEX, and CEX membranes in parallel batch mode provides exceptional productivity, exceeding resin-based multi-column chromatography (over 400 g/L/h vs. under 200 g/L/h).

Integrating Sartobind® Rapid A with process intensification strategies demonstrates a clear advantage of membrane-based mAb purification over traditional resin-based methods.