High-Throughput mAb Purification With Fibro Chromatography

By Linnea Troeng, Matthew Townsend, Oliver Hardick, Jinyu Zou, and Tuomo Frigard, Cytiva

Biomanufacturing is currently trending towards an increased number of monoclonal antibody (mAb) projects and smaller batch sizes, causing an increase in demands to screen more clones faster and improve the efficiency of research and process development (PD). Resin-based columns and liquid handling robots are often used to purify mAbs for high-throughput screening of lead candidates and optimize process conditions, but this setup requires a large footprint and capital-intensive equipment.

Rapid cycling, fiber-based chromatography (Fibro) enables substantially reduced purification times in research and PD. Fibro chromatography supports relatively high capacity, with residence times of seconds, and allows full chromatographic runs in a few minutes per cycle.

Here, we describe a technology for high-throughput, automated purification with the potential to reduce time-to-market. Discover how rapid cycling, using Fibro PrismA units together with an ÄKTA pure™ system and an autosampler, offers new opportunities in high-throughput purification for screening of lead candidates and process conditions.