10.05.23 -- Get The Most From Your Cell Culture

Our goal was to establish a host cell line with the desired growth characteristics and capability to seamlessly switch between cloning and production media. Learn how we confirmed cell performance within the cell line development workflow with the successful isolation of stable, high-producing clones from three model monoclonal antibodies.

Seasoned scientists and team leaders from Cytiva present the latest trends in cell culture technology as well as strategies to accelerate cell line development, media development, and raw material characterization. Additionally, they discuss tools and enablers for scale-up and explain the benefits of outsourcing process liquids and buffers.

When evaluating media with the goal of increasing titers, you need to ensure that protein quality is not negatively impacted. So, how can you reliably perform this process improvement? Use a Design of Experiment (DoE) and empirical approach. Read an example of how to do it.

ActiPro™ cell culture production medium is intended to help enhance recombinant protein production in fed-batch or perfusion processes. This work demonstrates the development of a fed-batch process for a CHO cell line producing a mAb. The results emphasize the importance of an appropriate feeding strategy to optimize cell growth and productivity.

Intensify your fed-batch process through reduced production duration without affecting the growth or titer profile. We show how the conventional N-1 seed train step was replaced by a high-density perfusion and how the production bioreactor can be seeded at a 10-fold higher seed density compared to conventional runs.