Newsletter | October 5, 2023

10.05.23 -- Get The Most From Your Cell Culture

Generating A Robust Host Cell Line For A Cell Line Development Platform

Our goal was to establish a host cell line with the desired growth characteristics and capability to seamlessly switch between cloning and production media. Learn how we confirmed cell performance within the cell line development workflow with the successful isolation of stable, high-producing clones from three model monoclonal antibodies.


Improving Efficiency In Upstream Process Development

Seasoned scientists and team leaders from Cytiva present the latest trends in cell culture technology as well as strategies to accelerate cell line development, media development, and raw material characterization. Additionally, they discuss tools and enablers for scale-up and explain the benefits of outsourcing process liquids and buffers.


Optimizing Cell Culture Media: Increase Titer With Maintained Protein Quality

When evaluating media with the goal of increasing titers, you need to ensure that protein quality is not negatively impacted. So, how can you reliably perform this process improvement? Use a Design of Experiment (DoE) and empirical approach. Read an example of how to do it.


Effects Of Feeding Strategy On CHO Cell Performance In Fed-Batch Cultures

ActiPro™ cell culture production medium is intended to help enhance recombinant protein production in fed-batch or perfusion processes. This work demonstrates the development of a fed-batch process for a CHO cell line producing a mAb. The results emphasize the importance of an appropriate feeding strategy to optimize cell growth and productivity.


Intensified, Improved Fed-Batch Production Process

Intensify your fed-batch process through reduced production duration without affecting the growth or titer profile. We show how the conventional N-1 seed train step was replaced by a high-density perfusion and how the production bioreactor can be seeded at a 10-fold higher seed density compared to conventional runs.