Gene Therapy And Stem Cell Transplantation Via MRI-Guided Intraparenchymal Delivery Into Brain Regions

This stem cell transplantation therapy involved a midline incision, unilateral or bilateral craniotomy, and the implantation of cannula guides into adult rhesus and cynomolgus macaques (weighing approximately 2.5 to 15 kg). The animals were anesthetized with Isoflurane, and a stereotactic frame compatible with MRI was carefully placed to ensure accurate targeting. Real-time MRI imaging was utilized throughout the procedure, enabling technicians to confirm that the treatment was delivered to the correct regions of the brain.

Additionally, the imaging allowed for real-time adjustments to flow rate, dosage, and other critical parameters to ensure optimal results. Two specific procedures are outlined in the case study below.