Perfusion processes have gained popularity due to the possibility to reach higher cell densities and extend the protein production phase. Increasingly, these processes are adopted at the early stages of process development to ensure that clones, media, and feeds that are taken forward are selected under the most representative conditions. To date, this method of processing has been a challenge at the microbioreactor scale due to the infeasibility of implementing a hollow fiber filter at this scale. Therefore, alternative methods of cell separation and media exchange are needed.
The Ambr® 15 Cell Culture system has been shown to provide improved results when compared to shake flask or shaking plate cultures from the high level of automation combined with reliable and independent process control for pH and DO. With low working volumes, the small scale stirred bioreactor vessels offer considerable advantages for screening of cell lines and media when running intensified processes, particularly in terms of costs.
In this application note, two perfusion mimic protocols have been established in the Ambr® 15 Cell Culture system to show how different approaches can be applied to facilitate understanding of intensified processes at the microscale.