A Unique Defined Pre-Coating - Free Culture Platform For Isolation And Expansion Of hMSC Towards Clinical Applications

Source: Sartorius

By Mira Genser-Nir, Sharon Daniliuc, Marina Teverovsky, Yuliya-Yael Miropolski, Maria Sharovetsky, Roni Hazan Brill, David Fiorentini

Human mesenchymal stem cells (hMSC) hold great promise as tools in cell therapy. Since hMSCs are rare in adult tissues, the isolated cells must be expanded in-vitro to generate sufficient cells number for clinical use.

The use of serum-free (SF), xeno-free (XF) culture system is an advantage in order to minimize the health risk of using xenogeneic compounds, and to limit the immunological reactions in-vivo. Under SF, XF culture condition, a coating step is usually required to enable cells attachment, spreading and proliferation in-vitro.

The coating procedure is an obstacle step for scale up towards cells therapy. Thus, having an optimal coating- free culture platform may provide efficient, user-friendly and economical hMSC manufacturing process.

In the present study, different combinations of treated plastic ware (uncoated) and SF, XF media were evaluated.

Results show that MSC NutriStem® XF (BI) together with Corning® CellBIND® (uncoated culture ware) is a superior platform for the expansion and proliferation of hMSC from a variety of sources.

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