FEATURED ARTICLES

• Complete Structural Elucidation Of Lipids In A Single Experiment Using Electron Activated Dissociation (EAD)

  Here, electron activated dissociation (EAD) on the ZenoTOF 7600 system is used for the complete structural elucidation of glycerophospholipids, sphingolipids, and acylglycerols in a single experiment.

• Orthogonal Fragmentation Mechanism Enables New Levels Of Metabolite Characterization

  Fragmentation is required to provide structural information about metabolites during drug discovery. Here, two orthogonal fragmentation techniques are compared to determine the in vitro metabolism of the drug darunavir.

• Tunable Electron Activated Dissociation (EAD) MS/MS To Preserve Particularly Labile Post-Translational Modifications

  Post-translational modifications (PTMs) are important players in a diverse group of functions that include protein conformation and signaling. This application note studies the site-localization of malonylated peptides using the SCIEX ZenoTOF 7600 system.

• Rapid And Reproducible Parallel Processing Of Charge Heterogeneity Analysis Of Protein Therapeutics

  In this technical note, we demonstrate parallel, highly reproducible capillary isoelectric focusing analysis using a multi-capillary separation approach to accelerate analysis time.

• Quantification Of Large Oligonucleotides Using High Resolution MS/MS

  Explore how to achieve sensitive, high-resolution analysis of large oligonucleotides, with the opportunity to perform both qualitative and quantitative analysis in a single run.

• Extending The Lower Limits Of Quantification Of A Therapeutic Oligonucleotide Through Microflow LC-MS/MS

  Here a solution is presented to widely recognized challenges with ionpairing reversed phase liquid chromatography (IP-RP LC) through a novel microflow LC-MS strategy.

• Metabolite ID And Relative Quantification Of Oligonucleotides In Plasma

  This technical note describes the identification, relative quantification and structural confirmation of the chain-shortened metabolites of a phosphorothioated oligonucleotide.

• Sensitive AAV Capsid Protein Impurity Analysis By CE Using Easy To Label Fluorescent Chromeo Dye P503

  We demonstrate an easy sample preparation and labeling scheme is described using commercially available fluorescent tag which doesn’t require buffer exchange nor dye cleanup for the low level detection of AAV8.

• Purity Analysis Of Adeno-Associated Virus (AAV) Capsid Proteins Using CE-LIF Technology

  We review a CE-SDS-LIF method for assessing the purity of AAV viral capsids utilizing FQ fluorescence dye labeling and LIF detector to provide an ultra-high sensitivity for in-process AAV product analysis with titer as low as 1X 10¹⁰ GC/mL.

• Purity Analysis Of Adeno-Associated Virus (AAV) Capsid Proteins Using CE-SDS Method

  We demonstrate the capability of the CE-SDS method for purity analysis of AAV viral proteins with easy sample preparation, excellent resolving power, good repeatability and linearity of absorbance response to sample concentration.

• Characterization Of Adeno-Associated Virus Capsid Proteins With Peptide Mapping

  Presented here is an approach for the characterization of AAV capsid proteins and associated post translational modifications (PTMs) using a bottom-up approach.

• Characterization Of Oligos, Related Impurities To Support Development

  This technical note describes the identification, relative quantification and structural confirmation of oligonucleotides and related impurities using molecule profiler software.

• Ultra-Sensitive Assay For Anti-Sense Oligonucleotide Quantification

  Learn about a new level in sensitivity for challenging analytes in matrix that opens doors for accurate bioanalysis and metabolism studies with specificity not achievable with orthogonal methods.

• Genome Sizing Methodology For Integrity Analysis Of AAV

  In this technical note, we describe the development of novel CE-LIF based methods for accurate determination of size purity of AAV genome.

• Comparative Analysis Of Intact AAV8 Capsid Proteins Derived From SF9 And HEK293 Cell Lines

  Presented here is a streamlined approach for the determination of the intact molecular weight of viral capsid proteins from AAV8.

• Determining Full, Partial, And Empty Capsid Ratios For AAV Analysis

  This technical note demonstrates a robust cIEF-based method for the separation and analysis of AAV full and empty capsids of different serotypes.

• Combining Qualitative Flexibility, Quantitative Power For Analytical Results

  The key to achieving robust analytical results lies in the combination of sensitivity, selectivity, and specificity. Recent technological advancements enable the most demanding sample types and workflows.

• Successfully Achieve High Numbers Of Peptide And Protein Identifications

  Collecting quality MS/MS at high acquisition rates is key to achieving high numbers of peptide and protein identifications. This study investigates the impact of large sensitivity gain on identification rates.

• Gene Therapy And Oligonucleotide Compendium

  Technical summaries and detailed protocols for purity testing, sizing, sequencing and expression analysis of oligonucleotide-based active agents as well as protein and lipid-based delivery systems.