Application Note

The Selection Of Depth Filtration Devices For Cell Cultures, Fermentation Broths And Cell Lysates

Source: Cytiva
Scientist Laboratory sample pipette GettyImages-1354172647

Solutions derived from cell cultures, fermentation broths, and cell lysates often contain large amounts of particulate load. The clarification of cell culture solutions is required to remove particles prior to further downstream processing, such as ultrafiltration or capture chromatography.

Particulates, such as cell debris, can vary in size and be difficult to filter using a standard membrane filtration device. Membrane filters are typically thin, highly porous materials with a narrow pore size distribution. This prevents particles larger than the rated pore size from passing through the filter. Particulate matter larger than the pore size will build up on the membrane surface during filtration, eventually clogging the open pores of the structure until no additional solution can be filtered. When filtering solutions that contain large amounts of particulate, a standard membrane filter can quickly block and need replacing.

Typically, devices containing depth media filters can offer more efficient filtration for solutions with heavy particulate loads. Depth media is thicker than filter membranes, so a range of particulate of different sizes can be trapped on both the surface and throughout the matrix of the filter media.

Browse depth filtration products and devices for the downstream processing of cell culture solutions, fermentation broths, and lysates.

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