Purification And Solubilization Of Membrane Proteins For Cryo-EM Studies: Screening Of Copolymer Nanodiscs To Optimize Stability

Membrane proteins are important targets for drug development because they are involved in many cellular processes and are easily accessible to drugs. However, they present additional challenges for structural analysis because of their amphipathic nature, complex architecture, and tedious expression and purification.
With the use of innovative solubilization approaches, such as synthetic copolymers and the NativeMP™ Platform, companies like Cube Biotech can more efficiently create soluble membrane proteins optimized for cryo-EM workflows to support structural biologists. In this application note, we describe how the application of dynamic light scattering (DLS) and nano Differential Scanning Fluorimetry (nanoDSF) provided by the Prometheus Panta helps membrane protein structural biologists rapidly find the best copolymers for their synthetic nanodiscs to ensure the highest atomic resolution for cryo-EM structures.
Download the full application note to learn more about how to optimize membrane proteins for cryo-EM.
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