Master Analytical SEC Running Conditions

By Jon Lundqvist, Scientist at Cytiva

Read these useful insights to answer the following questions:

• How do I calibrate my Superdex Increase or Superose Increase column?
• How do I best prepare my sample for SEC analysis?
• How large sample volume can I apply in an analytical run?
• What size sample volumes can I inject to the SEC column without losing resolution?
• Which buffers may I use with Superdex Increase and Superose Increase columns?
• What flow rate should I use?
• Can I use organic solvents?
• Is it possible to use DMSO on Superdex Increase columns?
• How do different additives affect my results?
• Do you have any recommendations to avoid IgG interactions with resin?
• How often do you recommend cleaning a SEC column?