Application Note

Isolation Of Individual Cells For Single-Cell Analysis

Source: Bio-Rad

By Joyce Lee

Biorad

Accurate isolation of individual cells is essential for single-cell analysis techniques. Here, we demonstrate a simple workflow to verify the reliability of the S3e Cell Sorter in sorting single cells. Using this method, we confirmed sorting accuracies of >94% and 100% for single cells and single beads, respectively.

Cellular heterogeneity, originating from stochastic gene expression, leads to difficulties in interpreting cell-to-cell variations in transcription and translation (Raj and van Oudenaarden 2008). Therefore, it is important to examine genomic, epigenomic, and transcriptional variation at the single-cell level. Flow cytometry combined with single-cell sorting allows researchers to identify and separate target cells for cell culture and other downstream analyses such as gene expression, transcriptome sequencing, and cell imaging. In this study, we demonstrate a method to verify the accuracy of single-cell sorting using the ZOE™ Fluorescent Cell Imager as an essential QC step and thus confirm that the S3e Cell Sorter is capable of reliably sorting single cells into 8-well culture strips.