Binol Scale-up And Repeatability Study

Jennifer L. Lefler and Jacquelyn Cole, Thar Instruments, Inc.

The pharmaceutical industry has shifted the paradigm of drug selection to require enantiopure formulations. In turn, the developments of chiral stationary phases (CSPs) have enable chromatographers to resolve racemic mixtures with increasing efficiency. Enantioselective liquid chromatography (LC) is recognized as a useful tool for the preparative isolation of stereoisomers across the spectrum of scales. However, chiral LC is a major consumer of resources, such as, time and organic solvents.

An attractive alternative to chiral LC is a closely related technology, which employs carbon dioxide as the bulk of the mobile phase, Supercritical Fluid Chromatography (SFC). SFC has extended the application of chiral chromatography as a means to resolve and/or isolate enantiomers by its highly tunable nature. The parameters of pressure and temperature in combinations with CSP and strong solvent selection can dynamically affect the resultant resolution. Supercritical CO2 also demonstrates lower viscosities than traditional HPLC mobile phases, thereby enabling geometric scalability from the analytical to the full spectrum of the preparative scale.

Thar Instruments implements its core competency of pumping supercritical carbon dioxide (CO2) in its chromatographic instrumentation offerings. The objective of this presentation is to spotlight the reproducibility of Thar's analytical and preparative SFC instruments via an exemplary purification of pharma-like material.

Product information on Prep SFC 350
Product information on SFC Method Station

SOURCE: Thar Instruments, Inc.