Application Note

Achieve Highly Sensitive And Precise Binding Affinity Measurements Without Disrupting Protein Conformation

NanoTemper - Monolith

Membrane proteins are essential for drug discovery due to their central role in cellular communication and signaling, making them key therapeutic targets for next-generation medicines. However, their unique properties pose significant challenges for biophysical characterization, particularly in maintaining stability and native conformation outside of biological membranes. Traditional detergent-based solubilization strategies often disrupt membrane protein structure and function, complicating the interpretation of experimental results.

This application note highlights the combined strengths of using the latest technologies from Cube Biotech and NanoTemper Technologies to overcome these challenges. Cube Biotech's NativeMP™ Technology utilizes detergent-free synthetic copolymers to stabilize membrane proteins in their native environment. The Monolith X instrument from NanoTemper Technologies employs Spectral Shift and TRIC technologies to directly measure biomolecular interactions in solution.

Download the full application note to learn how coupling NativeMP™ Technology with the Monolith X instrument empowers researchers to obtain highly sensitive and precise binding affinities while preserving the protein's native conformation during measurements. Discover how this combination can advance your membrane protein interaction studies and accelerate new therapeutic discoveries.

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