Practical Guide: Quality Control Strategies For Membrane Proteins

A practical guide with insights from Dr. Melanie McDowell from the Max Planck Institute of Biophysics

Achieving a stable, high-quality membrane protein sample is the cornerstone of successful cryo-EM. Without rigorous quality control during purification, even the most advanced microscopes won't yield the results you need. This guide, featuring detailed advice from Dr. Melanie McDowell, Research Group Leader at the Max Planck Institute of Biophysics, provides a deep dive into the practical strategies that ensure your membrane protein samples are cryo-EM ready.

From navigating the complexities of detergent exchange and nanodisc assembly to optimizing lysis methods and understanding micelle size variability, Dr. McDowell offers battle-tested solutions. Learn how to troubleshoot common issues and refine your protocols for optimal sample behavior. Explore the full article for a comprehensive approach to membrane protein quality control.