High-volume laboratories must keep analytic instruments running reliably while producing quality data. This means laboratory managers and technicians must ensure their instruments are performing optimally around the clock. Filtering samples before injection into a chromatography instrument is one of the primary ways that an analyst can protect their column and instrument from unnecessary wear and excess downtime. Filtration of both the sample and mobile phase prior to analysis helps increase the lifespan of chromatography columns, reduce overall instrument wear, and remove any particles that may interfere with the chromatogram.
Of the four common causes for HPLC column failure – plugging, voids, absorbed sample, and chemical attack – plugging is the most frequently encountered by chemists or analysts. Injection of samples containing particulates will eventually block the column inlet and column packing, cause high column back pressure, and shorten the lifetime of the column.
This scientific brief reviews how high-performance syringe filters protect and extend the life of analytical HPLC and UHPLC instruments and columns.