High-Throughput Characterization Of RNA Using The BioPhase 8800 System

By Zhichang Yang, Mario Pulido, Jane Luo, Tingting Li and Sahana Mollah, SCIEX

The vaccine has been the most successful medical approach for preventing and controlling the spread of infectious diseases, including the pandemic-causing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Multiple Covid-19 vaccines have been authorized for emergency use, including mRNA vaccines that have been highly effective in controlling SARS-CoV-2. Relative to vaccines that utilize inactivated viruses, purified antigenic subunits of pathogens or DNA to induce immunity, mRNA vaccines offer advantages in safety, efficacy, development speed and large-scale production capability.

The development and quality control phases of producing therapeutic RNA-based molecules each require extensive characterization of the molecules produced. Accelerated analysis of characterization can save significant amount of time which is crucial for the fast RNA based vaccine development. Here we demonstrate how a multi-capillary capillary electrophoresis (CE) system, BioPhase 8800 system, is used to efficiently separate a wide range of RNA-based molecules by size and demonstrates the multiplex capability for RNA characterization.