By Mariko Alexander and Angela Ra
One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. Proper optimization and validation is essential for successful one-step multiplex RT-qPCR. This initial investment of time up front ensures that resources and samples are not wasted producing suboptimal data later on. Optimization is particularly important for labs that repeatedly analyze the same targets, where the assay conditions will be used for many downstream experiments. What should you consider to ensure you are choosing the right reagents for your needs?