Many techniques in Life Science research rely on efficient sample preparation methods. Concentration and purification of samples are key steps in the workflow that can greatly affect the outcome of the experimental results. Pall has increased the range of AcroPrep 24-well filter plates offered to help streamline membrane selection and provide effective solutions for multiple sample preparation needs. The new AcroPrep 24-well filter plates include membranes for ultrafiltration (molecular weight cut off ranging from 1 to 100 K), microfiltration (pore sizes ranging from 0.1 to 10 μm) and macrofiltration/particle filtration (30 – 40 μm non-woven media). This document reports the data generated by ultrafiltration of proteins of known size with AcroPrep 24-well filter plates. Microfiltration and macrofiltration using the AcroPrep 24-well filter plates with relevant pore size were examined using calibrated latex beads mimicking relevant material diameter.