Application Note

Acroprep™ 24-Well Filter Plates With Depth & EKV Media Or EKV Media For Clarification Of Mammalian Cell Cultures

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Centrifugation and filtration have been widely accepted as techniques required for clarifying complex cell cultures  to recover extracellular proteins such as monoclonal antibodies (mABs). These steps can be time consuming and costly for labs growing their cultures in 24-well culture plates. This document offers an alternative to the use of  centrifugation/filtration/flocculation to clarify mammalian cells cultures and describes assessment of Pall’s newly  developed AcroPrep 24-well clarification and sterilization plate and AcroPrep 24-well sterilization plate for clarification of mammalian cell cultures (2 to 26 x 106 cells/mL) and recovery of proteins present in the supernatant.

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