By Udara Dharmasiri, Georgius de Haan, David Rich, and Kristoffer Bolen, Pall Corporation
The demand for high purity plasmid DNA continues to increase in molecular biology research. Advances in cloning techniques have greatly increased the number of samples, necessitating high-throughput small-scale plasmid preparation.
Various applications such as drug delivery, preclinical studies, and production of DNA vaccines require different amounts of high quality plasmid DNA. Typically, selection and optimization of suitable plasmid DNA for these applications start with small scale cultures of 1-1.5 mL volume. Plasmid DNA purified from these bacterial cultures provides material for clone verification. Further characterization is often accomplished by techniques such as polymerase chain reaction (PCR), restriction enzyme digestion combined with gel analysis, and Sanger sequencing.
Here we describe the use of AcroPrep Advance 96-well Long Tip Filter Plate for Nucleic Acid Binding (Pall NAB plate) for small scale plasmid DNA purification. These plates contain a unique quartz-based media suitable for nucleic acid isolation and purification and a new long tip geometry that minimizes hanging-drops and crosstalk. Combined, these improvements translate into higher throughput, increased sensitivity and signal-to-noise ratios, and reduced processing times.