Supporting Expansion Of Human Mesenchymal Stem Cells Using Thermo Scientific Nunc Cell Factory Systems

This poster illustrates how Thermo Fisher Scientific can support customers in their bioprocess of choice for the expansion of hMSCs on a Thermo Scientific™ Nunclon™ Delta surface, using either small-scale cultureware or larger-scale Thermo Scientific™ Nunc™ Cell Factory™ systems^a (CF). For each vessel type, data is presented with either classic, 10% serum-containing medium or with Gibco™ MesenPRO RS™ Medium^b, containing 2% serum. Overall, we report consistent performance between Nunclon Delta-treated T75 flasks^c and 1-tray Nunc Cell Factory systems^a using either classic medium or MesenPRO RS Medium^b. The comparable performance between the small-scale and the larger-scale culture vessels is further highlighted by the cell yield obtained per cm² which differed by only 10% between T75 flasks^c and 1-tray Cell Factory systemsa for each media type. In addition, overall cell viability was greater than 96%. We also report true scalability of hMSC cultured in classic, serum-containing medium by increasing the surface area contained within the Nunc Cell Factory system^a from a 1-tray vessel to a 10-tray vessel: When seeded at a density of 2000 cells/cm², eight million cells (8.1 E06) were harvested from a 1-tray Cell Factory system^a following 4-day culture. Following expansion to a 10-tray Cell Factory system^a using the same culture paradigm, 80 million cells (8.27E07) were harvested. This 10-fold increase in yield corresponds to the 10-fold increase in available surface area between a 1-tray and a 10-tray Cell Factory systema. The scalability of expanding hMSCs in classic, serum-containing medium within Nunc Cell Factory systems^a is reflected by the consistent growth rate of hMSC and the retention of the cells’ differentiation potential when expanded from a 1-tray to a 10-tray Cell Factory system^a.