The Pan-Enterovirus reagent has an atypical staining pattern in the cytoplasm that should appear speckled and perinuclear. In order to reduce the incidence of non-specific staining associated with enterovirus testing, it is suggested that the user pre-incubate the sample slide-well with PBS wash buffer containing 1% casein or 1% Difco-Bacto Skim Milk for 15-30 minutes at 37°C. This step eliminates most non-specific staining, however it will not affect specific enterovirus activity. After the milk pre-incubation step is complete, it is suggested that the user rinses thoroughly, then proceed with normal testing protocol.
Chemicon International, 28835 Single Oak Dr., Temecula, CA 92590 Phone: 1-800-437-7500 Fax: (909) 676-9209