Safely Separate Microcarriers And Harvest Cells In A Closed System
Current techniques used to separate adherent mammalian cells from microcarrier beads include: sedimentation using conical or inclined settlers, centrifugation, acoustic resonance, spin filtration, and microfiltration. These techniques often use sophisticated equipment, requiring significant capital expenditure as well as routine maintenance and between-use cleaning and sterilization. Until now, single-use options were restricted to disposable spin filters and hollow-fiber/microfiltration systems.
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