Tags simplify purification and enable scientists to use standard protocols while also improving the yield, purity, and solubility of the protein of interest.
ÄKTA start eliminates the hassles of manual protein purification handling, such as loading large sample volumes and continually monitoring UV readings, and can also give you consistency of yield and purity.
This handbook describes how to design a protein purification procedure, how to select and combine chromatography methods and purification formats, and how to monitor and evaluate protein purification.
Find recommendations for immobilization of histidine-tagged molecules (ligands) by capture to an anti-histidine antibody covalently coupled to a sensor chip surface.
Each of the steps and products selected during your purification and analysis workflow will influence the results in terms of recovery, purity, and analytical quality but will also open opportunities to save time and money.
