11.17.22 -- Improve And Intensify Your Upstream Processes

Identifying some key items about your cell line from the beginning, such as the nutrients it needs over the culture time, impact of metabolites, and critical osmolality, can help to rapidly design a simple daily feeding regimen to perform a production scale. This article describes a straightforward way to achieve a high-performing fed-batch process.

See how the different unit operations in a laboratory-scale monoclonal antibody (mAb) process can be connected and integrated into a semicontinuous process. The mAb process consists of perfusion cell culture, continuous capture chromatography, viral inactivation (VI), post VI filtration, batch polishing chromatography, and a final pH adjustment step.

Intensify your fed-batch process through reduced production duration without affecting the growth or titer profile. We show how the conventional N-1 seed train step was replaced by a high-density perfusion and how the production bioreactor can be seeded at a 10-fold higher seed density compared to conventional runs.

Perfusion processes enable continuous operation over extended periods of time by constantly providing fresh nutrients for the cells and simultaneously removing spent media and waste products. This study demonstrates a connected mAb perfusion cell culture process using Xcellerex XDR 50 integrated with the Xcellerex Automated Perfusion System.