Buffer preparation can be both time-and space-consuming and can easily become a challenge in biomanufacturing. In-line preparation of buffers from highly concentrated, single component stock solutions saves both time and storage space. Compared with preparing one concentrate per buffer formulation, many different buffers can be prepared from the same stock solutions using GE Healthcare’s Inline Conditioning system.
With the Inline Conditioning system, buffers can be formulated just-in-time in an automated manner. The system can be used off-line as a stand-alone central buffer preparation station to feed different processes in the facility or in-line connected to a chromatography column using a built-in chromatography functionality. Compared with preparation of buffers as a separate operation, integration of buffer preparation with the chromatography step both saves time and reduces facility footprint. Feedback control of the preparation process ensures accurate formulation of the final buffer, and the automation enables high consistency between batches. Compared with traditional buffer preparation, in-line conditioning can help increase efficiency in buffer production for biomanufacturing applications.