Poster

Development Of An Intensified Fed-Batch Process Utilizing N-1 Perfusion And ambr®15

Source: Catalent

While traditional fed-batch bioreactor process development has been well defined using methods such as shake flasks and bench-scale bioreactors, there is a desire to intensify the fed-batch process strategy to increase efficiency and productivity.  Utilization of perfusion during the N-1 stage allows for inoculation of the production bioreactor at a very high cell density. Learn how to combine N-1 perfusion with the ambr®15 bioreactor system to create a powerful tool for rapid development of a highly efficient cell culture process.

Key Learning:

  • How N-1 perfusion can increase product titer and/or decrease production time
  • How ambr®15 can be utilized to rapidly screen a variety of media conditions using a DOE approach
  • How N-1 perfusion and ambr®15 can be utilized together to quickly identify top conditions to scale up to 2L bioreactor

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